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Thursday, December 3, 2020 | History

5 edition of Techniques for Semen Evaluation, Semen Storage, and Fertility Determination found in the catalog.

Techniques for Semen Evaluation, Semen Storage, and Fertility Determination

Murray R. Bakst

Techniques for Semen Evaluation, Semen Storage, and Fertility Determination

  • 232 Want to read
  • 16 Currently reading

Published by Poultry Science Assoc .
Written in English

    Subjects:
  • Medical

  • The Physical Object
    FormatPaperback
    ID Numbers
    Open LibraryOL8528063M
    ISBN 100964981114
    ISBN 109780964981119
    OCLC/WorldCa38860709

    Evaluation of the percentage of spermatozoa exhibiting progressive forward motility is the most commonly used indicator of both viability and fertility of porcine semen. Provided that the semen sample has been prepared properly for microscopic evaluation, motility and viability are closely related - that is the percentage of motile sperm cells. An integral part of the infertility evaluation of a couple is the examination of the man’s semen. Semen analysis is the most important screening test for evaluating male infertility and should be performed times during the couple’s infertility evaluation. Semen analysis is a laboratory procedure in which the ejaculate is examined to determine the.   The article reviews methods used for in vitro evaluation of sperm, with particular emphasis on frozen-thawed stallion sperm. The techniques, limitations of the methods and correlations with fertility results are discussed. Very few studies have tried to find correlation between fertility of frozen stallion semen and laboratory tests. It is difficult and expensive to inseminate an adequate. 1 day ago  Compiled & Edited by-DR RAJESH KUMAR SINGH,JAMSHEDPUR,JHARKHAND, INDIA, ,[email protected] Among the myriads of techniques devised for the genetic improvement of animals, artific.


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Techniques for Semen Evaluation, Semen Storage, and Fertility Determination by Murray R. Bakst Download PDF EPUB FB2

Techniques for Semen Evaluation, Semen Storage, and Fertility Determination Paperback – January 1, by Murray R. Bakst (Author), H. Cecil (Author)Authors: H. Cecil, Murray R. Bakst. Techniques for Semen Evaluation, Semen Storage, and Fertility Determination Murray R.

Bakst, Helene C. Cecil Poultry Science Association, - Poultry - 97 pages. Techniques for Semen Evaluation, Semen Storage and Fertility Determination [CD-ROM].

Version And Fertility Determination book. Paul, MN: Midwest Poultry Federation. Interpretive Summary: Technical Abstract: This procedure provides the quantitative determination of the rate of sperm penetration of the perivitelline layer (PL).

This PL sperm penetration assay is. Techniques for Semen Evaluation, Semen Storage, and Fertility Determination. Edited by M. Bakst and H.

Cecil. تکنیک‌های ارزیابی Semen، ذخیره Semen و تعیین باروری. ویرایش شده توسط م. Bakst و اچ. Cecil. Keywords: comparative sperm evaluation, fertility estimation, in vitro methods, semen analysis, sperm quality. Introduction. Over the past decade, we have experienced an explosive development of in vitro assays to determine sperm intactness and measurement of sperm function that helped andrological diagnosis and the optimization of semen.

Precision of the evaluated methods. It is necessary to establish that a method is repeatable before comparing two measurements for reproducibility (Petrie & Watson, ).Repeatability of boar semen concentration assessment depends on instruments and procedures, for example CV for instruments FACS, HEMO, Photo C, SpermVision, UltiMate and SP were.

Techniques for Semen Evaluation, Semen Storage, and Fertility Determination. 2nd edition. Paul, MN: The Midwest Poultry Federation. Proceedings of the XXII World's Poultry Congress Book of Abstracts. (abstract). SPERM STORAGE IN THE HEN'S OVIDUCT - (). bility and Mitochondrial Membrane Potential in Fixed Semen Samples.

Cytometry Part B ; 94B: – INTRODUCTION Accurate evaluation of the quality of a semen sample or the fertility of a stallion is of utmost importance for the equine industry (1). Techniques for Semen Evaluation analysis of sperm allows for the evaluation of numerous functional. Significance of Semen Quality.

The goal of all attempts to measure semen quality is to ensure that the subsequent fertility of the semen will be in the normal range or, in other words, to make maximum use of proven bull while maintaining optimum fertility. The terms “semen quality” and “fertility” are. Methods of semen collection and artificial insemination AI in poultry, requirement for.

and Fertility Determination book, methods of liquid and frozen storage of avian semen and evaluation of spermatozoa after storage for fertilizing ability are reviewed. Frozen storage of semen from non-domestic birds is also briefly discussed.

Published by Elsevier Science B.V. The SQF values of individual ganders varied from to Means for ejaculate volume, sperm concentration, live and morphologically normal spermatozoa, motility, pH, and SQF were mL, × 10 6 /mL, %, %,andrespectively ().Very large variations among ganders were found for all semen parameters except for pH.

Extended, chilled semen is frequently transported for insemination, providing a useful alternative to either freezing or immediate use. To maintain temperature, special containers are made just for semen transport.

The "Equitianer" pictured at right contains coolant cans and insulation to keep the semen sample cool for overnight shipping. The phenotype-linked fertility hypothesis suggests that the honesty of these signals may be maintained through a link with fertility.

Indeed, in some recent studies, bright coloration has been linked with better fertility and also indicates semen characteristics that influence fertility.

Techniques for Semen Evaluation To assess the potential fertility of a male and provide diagnostic information in cases of infertility Semen has been collected from a very large number of creatures.

In dairy cattle, chickens and turkeys, semen collection, processing and insemination is a large industry, and has been indispensible in genetic improvement programs. The general aim of semen evaluation is to assess the prospects of fertility of individual stallions or individual semen samples such as ejaculates or doses of fresh, cooled-transported, frozen/thawed semen).

1 – 10 The target of semen evaluation is to clarify if quantitative and qualitative spermatological parameters are in compliance with the minimum requirements fixed for biological. frozen semen. Limited comparisons of fertility showed that the conception rate at first service was 17% higher when semen was collected with an AV.

However, other reports have not found a significant difference in fertility with semen collected by thes e two different methods.

Recent advances in the. The schematic for the evaluation of storage techniques is on the left in green, and the schematic for evaluation of semen separation techniques is on the right in blue. Aliquots from each sample were divided into treatment groups.

After processing, DNA integrity in each group was assessed by SCD. For semen separation technique evaluation, sperm. Evaluation of the effect of laboratory methods on semen analysis and breeding soundness examination (BSE) classification in stallions Kristina Whitesell 1, Darko Stefanovski, Sue McDonnell, Regina Turner* Department of Clinical Studies, New Bolton Center, University of Pennsylvania, School of Veterinary Medicine, W.

Street Rd., Kennett Square, PA, Sperm analysis is one of the first methods to analyze and assess male fertility. In the process of basic semen analysis the motility, shape and number of sperm is examined. Sperm analysis is given in a private room in the clinic.

It is important to refrain from ejaculation for days before sperm sample is given in the clinic. Bakst, M.R. and Cecil, H.C. () Techniques for semen Evaluation, semen storage, and fertility determination. Bakst and Cecil editors. Published by.

Storage temperature of 5°C was reported to be optimum as, no significant differences were observed in candling fertility (85 vs. 82%) of Turkey hens AI with the semen diluted with BPSE () with unstored semen or semen held at 5°C for 18 h whereas higher storage temperatures have shown loss in fertility Dilution studies with IMV diluents.

Storage: Frozen semen are stored at ˚C in liquid nitrogen for transportation anywhere AI is desired. Evaluation of fertility of frozen semen post thawing A variation in the number of services per conception in different lactations has been reported to be due to difference in fertility of frozen semen.

Canine Semen Analysis examines factors such as the history of the dog, his reproductive system, general physical exam, hormonal evaluation, libido determination, assessment of diseases and more. Semen analysis is part of ensuring breeding soundness, which is based on the three distinctive fractions of semen ejaculated by the dog.

fertility during hypothermic storage of liquid semen (Ball et al., ). The survival of ejaculated sperm in seminal plasma alone is limited to a few hours. To maintain sperm for longer periods and to cool or cryopreserve semen, dilution with a protective solution is necessary (Ax et al., ).

However ram semen fertility in egg. Endocrine Diagnostics and Therapeutics for the Stallion with Declining Fertility Section V — Semen Collection and Evaluation Evaluation of Raw Semen Urospermia and Hemospermia Transmission of Diseases Through Semen Advanced Semen Test for Stallions Preservation of Genetics from Dead and Dying Stallions of raccoon dog semen, this type of research, enabling determination of changes at the molecular level in cells with seemingly normal morphology, has not been conducted.

The results of such research could form the basis for improving methods of storage and preservation of raccoon dog semen or for the devel-opment of new methods. with semen evaluation, the better we can determine the potential fertility of the ejaculate.

AKC Canine Health Foundation, Inc. • Arco Corporate Drive, Suite • Raleigh, NC •. Book: Larger Work Subtype: Other Government Series: Larger Work Title: Techniques for Semen Evaluation, Semen Storage, and Fertility Determination: First page: Last page: Google Analytic Metrics: Metrics page: Additional metadata about this publication, not found in other parts of the page is in this table.

Review Article COLLECTION AND EVALUATION OF CANINE SEMEN-A REVIEW M. Arokia Robert1*, G. Jayaprakash2, Mayur Pawshe3, T. Tamilmani4, and M. Sathiyabarathi5 1&2MVSc Scholar, College of.

Methods: male patients undergoing assisted reproductive technologies (ART) in our clinic were surveyed about their age, BMI (body mass index), ejaculation frequency, nutrition, sports, sleeping habits and social behavior. Semen samples were collected and evaluation of semen parameters according to MSOME and WHO criteria was performed.

Semen Evaluation Semen must be evaluated as soon as possible after collection, because changes in temperature, exposure to light, and exposure to any type of chemicals, lubricants etc. can change sperm motility and adversely affect fertility.

A general sperm count as part of a fertility evaluation should include the total density or count (20 million per ml or above), and the motile density (8 million per ml or higher). The motile density is perhaps the most important part of the semen analysis, as it reports the total number of sperm thought capable of progressing from the site of.

Semen collection & evaluation enables us to check the sample for the following: Volume: the volume collected affects the fertility in that a lower volume can contain fewer sperm.

Clarity: sperm cells make the semen look milky. If the semen collected is completely clear, there are no or very few sperm present.

Because semen analysis is the primary diagnostic tool in male fertility investigations, we assessed its performance in clinical laboratories. Werandomly surveyed United States hospital laboratories to determine the factors that predict demand for semen analysis and to assess the technical caliber and quality of semen testing performed.

Standard semen parameters are limited in their capacity to distinguish subfertile boars and to assess storage influences on liquid preserved boar semen. The evaluation of sperm chromatin structural integrity could have potential as a diagnostic tool in AI practice.

This study assessed whether the de. Artificial insemination (AI) is the manual transfer of semen into the female’s vagina. Basically it is a two step procedure: first, collecting semen from the male []; and second, inseminating the semen into the female [].In poultry, depending on the objectives and goals of the farm or laboratory, there may be intervening steps such as semen dilution, storage, and evaluation.

The various tests for semen quality are discussed in detail as is custom selection of semen. Part Three explains insemination techniques for dairy and beef cattle, inseminator training, pregnancy determination in cattle, conception rates, and breeding problems.

The exercise on "Embryo Transfer and Related Practices" explains the advances and Reviews: 2. For prediction of fertility and for improving freezing methods, it is important to develop reliable techniques to assess the quality of semen in vitro. Many methods exist and are used, but not many studies have examined or showed the connection between laboratory test results and fertility of frozen-thawed stallion semen [ 4 ], [ 5 ].

Semen quality is considered as methods for sperm quality evaluation to identify males of different fertilizing abilities. It is shown that sperm quality assays can be used to predict fertilizing ability and to select the 'best breeders' from a flock of chickens or turkeys when artificial insemination is employed.

ejaculate. Semen fertility must also be extended over days because raw semen is only fertile for hours after collection. Most semen extenders are classified into short ( days), medium () or long-term extenders ( days) from the day of collection.

The proper extension of semen is a critical step to achieving success with AI. Part of the Methods in Molecular Biology book series (MIMB, volume ) Abstract Ravie O () Effect on fertility of storing fowl semen for 24 h at 5 degrees C in fluids of different pH.

J Reprod Fertil – Lake PE () Studies on the dilution and storage of fowl semen. Reproduction –35 Google Scholar. Bakst MR, Cecil HC () Sperm viability. I. Nigrosin/eosin stain for determining live/dead and abnormal sperm counts.

In: Bakst MR, Cecil HC (eds) Techniques for semen evaluation, semen storage, and fertility determination. The Poultry Science Association Inc, Savoy (Illinois), pp 29– Google Scholar.Because semen analysis is the primary diagnostic tool in male fertility investigations, we assessed its performance in clinical laboratories.

Techniques for Semen Evaluation, Semen Storage.